Integrated risk evaluation for oxygenated polycyclic aromatic hydrocarbons (oxy-PAHs) in atmospheric environment

2022 Fiscal Year Final Research Report

• Project/Area Number: 20K10452
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 58020:Hygiene and public health-related: including laboratory approach
• Research Institution: University of Shizuoka
• Principal Investigator: Misaki Kentaro 静岡県立大学, 看護学部, 助教 (40468591)
• Co-Investigator(Kenkyū-buntansha): 井上 健一郎 静岡県立大学, 看護学部, 教授 (20373219) ; 高村 岳樹 神奈川工科大学, 工学部, 教授 (50342910)
• Project Period (FY): 2020-04-01 – 2023-03-31
• Keywords: 大気汚染 / oxy-PAHs / アレルギー増悪作用 / 酸化的損傷 / プロモーション活性 / RNA測定

Outline of Final Research Achievements

Aiming to elucidate integrated risk evaluation of oxy-PAHs in atmospheric environment, it was confirmed that TGF-β decreased significantly with BPO and NA in Foxp3-recombinated Treg-like cells, and IL-8 increased and several genes was induced with several quinones and 3-NBAO in human lung epithelial cells. Lung inflammation for mite antigen sensitization was exacerbated with 5,6-ChQ in mouse. Weak mutagenicity of several oxy-PAHs, and the variation of genes related to cell connection, differentiation, proliferation, etc. by BPO and 3-NBAO with prominent promotion activity in mouse embryo fibroblast cells were also confirmed. The improvement of detection system in these activities, and the identification of genes and the elucidation of signal transduction related to these activities are future issues.

Free Research Field

環境毒性学

Academic Significance and Societal Importance of the Research Achievements

oxy-PAHsの大気環境におけるアレルギー増悪・発ガンへの影響について、寄与物質や作用機序に関する知見はほとんど明らかにされていない。今回、複数の実験系による各種毒性評価を実施し、複数の活性物質が見出された。さらに各種毒性の検出系改良、活性物質の同定、各種遺伝子誘導、シグナル経路に関する知見を蓄積することで、国内外における大気環境汚染に伴うアレルギー疾患や肺ガン発症リスクの予測・低減に将来的につながっていくものと考える。