Establishment of a Novel Evaluation System for the Effect of Transdermal Exposure to Metal Oxide Nanoparticles by a Vitrigel-mediated Co-culture System

2023 Fiscal Year Final Research Report

• Project/Area Number: 20K10453
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 58020:Hygiene and public health-related: including laboratory approach
• Research Institution: Saitama Medical University (2023); Jikei University School of Medicine (2020-2022)
• Principal Investigator: Yogosawa Shingo 埼玉医科大学, 保健医療学部, 准教授 (70381936)
• Project Period (FY): 2020-04-01 – 2024-03-31
• Keywords: 金属酸化物 / ナノ粒子 / 細胞老化

Outline of Final Research Achievements

While demand for metal oxide nanoparticles (MONPs) such as zinc oxide nanoparticles (ZnONPs) is increasing, there have been reports of inflammation induction. Skin sensitization studies using mice were conducted using the LLNA-BrdU method with cell proliferation response in local lymph nodes as an indicator, but the effect of ZnONPs application to mouse skin was not significant. In in vitro studies, we found decreased expression of interleukin-8 and bleomycin hydrolase in response to induction of cellular senescence by exposure to ZnONPs. In mouse model experiments, no significant decrease in expression of involucrin or bleomycin hydrolase was observed, but a tendency toward keratin thickening was observed. In addition, important basic data for the construction of an in vitro evaluation system for MONPs was obtained.

Free Research Field

予防医学

Academic Significance and Societal Importance of the Research Achievements

利用が年々高まっている酸化亜鉛ナノ粒子の曝露影響について、 in vivo、in vitro両面からの基礎データを示すことができた。特にヒトの角化細胞由来HaCaT細胞と免疫反応が樹状細胞と同等といわれるTHP-1細胞を共培養した際にIL-8の誘導増強がみられるというデータは細胞老化誘導による攪乱の可能性を示す新しい知見である。酸化亜鉛ナノ粒子のような金属ナノ粒子は大変有望な物質材料であり、現在も改良・開発が進んでいる。本研究のさらなる進展により、安全性の高い高機能物質の開発に寄与できると考えられる。