Effects of influenza vaccines on cell-mediated immunity.

2023 Fiscal Year Final Research Report

• Project/Area Number: 20K10458
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 58020:Hygiene and public health-related: including laboratory approach
• Research Institution: Hyogo Medical University
• Principal Investigator: Otani Naruhito 兵庫医科大学, 医学部, 講師 (10561772)
• Co-Investigator(Kenkyū-buntansha): 奥野 寿臣 兵庫医科大学, 医学部, 非常勤講師 (10221152) ; 島 正之 兵庫医科大学, 医学部, 教授 (40226197) ; 中嶋 一彦 兵庫医科大学, 医学部, 准教授 (40340955)
• Project Period (FY): 2020-04-01 – 2024-03-31
• Keywords: インフルエンザワクチン / 細胞性免疫 / 抗体

Outline of Final Research Achievements

IFN gamma is considered a key moderator of cell-mediated immunity. However, little is known about its association with granzyme B, which plays an important role in the effector function of cytotoxic T lymphocytes (CTLs). In the present study, we collected blood samples from 32 healthy adults before and after vaccination with inactivated influenza vaccine in 2017/18 to measure the levels of IFN gamma and granzyme B, which play a role in cell-mediated immunity, and hemagglutination inhibition (HAI) antibody, which plays a role in humoral immunity. The levels of IFN gamma and granzyme B were significantly correlated both before and after vaccination. Furthermore, the post-vaccine fold increases in Interferon gamma and granzyme B levels were significantly correlated.

Free Research Field

感染症の疫学

Academic Significance and Societal Importance of the Research Achievements

インフルエンザの免疫にはＣＤ４陽性Ｔ細胞とＣＤ８陽性Ｔ細胞の両方が重要と考えられ、CD8陽性T細胞のうちＣＤ８陽性cytotoxic T lymphocytes (CTLs)の活性化に重要な蛋白質の１つがgrnzyme Bであることが知られており、ワクチン接種とグランザイムBの関係についての研究もおこなわれている。しかし、IFN gammaとGranzyme Bの関連性を見た研究はあまりない。そのため、本研究において不活化インフルエンザワクチンの細胞性免疫への影響を評価するためワクチン接種前後のIFN gammaとgranzyme bを測定しこれらの関連性をみることは重要であると考えられる。