Development of electrochemiluminescence determination system with molecular recognition for forensic toxicological analysis

2023 Fiscal Year Final Research Report

• Project/Area Number: 20K10553
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 58040:Forensics medicine-related
• Research Institution: Shinshu University
• Principal Investigator: Takahashi Fumiki 信州大学, 学術研究院理学系, 准教授 (40754958)
• Co-Investigator(Kenkyū-buntansha): 金 継業 信州大学, 学術研究院理学系, 教授 (40252118)
• Project Period (FY): 2020-04-01 – 2024-03-31
• Keywords: 電気化学発光 / 分子インプリントポリマー / 薬毒物分析 / オンサイト分析

Outline of Final Research Achievements

Electrochemiluminescence (ECL) has attracted attention as a highly sensitive analytical method. However, the selectivity of the technique has been low, and the analytical application has been limited to actual samples, such as biological samples.
In this study, we focused on the molecularly imprinting polymer (MIP) method and attempted to establish a specific ECL detection method for medicinal toxicants analysis. We utilized the specific recombination properties of MIPs synthesized using the target chemical species as a template. Subsequently, we have developed an ECL method for forensic analysis, which requires strict determination. A simple and selective detection protocol for regulated toxicants was proposed.

Free Research Field

分析化学

Academic Significance and Societal Importance of the Research Achievements

本研究課題で得られたMIPによる目的化学種の選択濃縮ECL検出による高感度分析法の融合は，ECL法を今までにない新しい選択的な薬毒物検出法へ展開できることが期待された。特に，鋳型分子を用いる関係で合成の際に課題となる規制薬毒物分析への応用について，MIPに関する基礎的な知見が得られ，法医化学および分析化学領域の適用範囲を拡張できた。また，ポリマー内への分析対象化学種の再吸着挙動の詳細な解析が行われ，従来まで検討例が限定的であったMIP中の分子認識空間の特性が評価されたことで，分析結果の信頼性の向上が図られた。