Effects on immune function and intestinal organic acids with long-term use of enteral nutrition products in medically managed children.

2023 Fiscal Year Final Research Report

• Project/Area Number: 20K10937
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 58070:Lifelong developmental nursing-related
• Research Institution: Yamaguchi University
• Principal Investigator: Hoshide Madoka (梶本まどか) 山口大学, 医学部, 講師(連携講座) (30610878)
• Co-Investigator(Kenkyū-buntansha): 松重 武志 山口大学, 医学部附属病院, 講師 (60528941) ; 井上 裕文 山口大学, 医学部附属病院, 助教 (70650604)
• Project Period (FY): 2020-04-01 – 2024-03-31
• Keywords: IESS / 腸内細菌叢 / 医療的ケア児

Outline of Final Research Achievements

The purpose of the study was to compare children with developmental epileptic encephalopathy (IESS, Lennox-Gastaut syndrome, intractable epilepsy with frequent epileptic seizures), which may be an underlying disease in children with medical care who regularly attend Yamaguchi University Hospital, with healthy children and to determine whether there are differences in the intestinal microbiota before or after treatment compared with healthy children. We decided to revise the purpose of the study. Samples were collected non-invasively from (1) healthy group (26 cases), (2) pre-treatment group (12 cases), (3) effective treatment group (10 cases), (4) and relapse group (5 cases) and analyzed for diversity using biologically relevant amplicon sequencing data analysis (RDP/microbe identification database) and QIIME II. No significant differences were found in either α or β diversity analysis.

Free Research Field

小児神経

Academic Significance and Societal Importance of the Research Achievements

既報では門レベルではIESSで治療前群でverrucomicrobiaが有意に増加する（p=0.001）、てんかん患者群の腸内（成人）ではproteobacteriaとFusobacteria phylumが健常群と比し有意に検出されるとの報告があるが、本研究、乳児に限っては発達性てんかん脳症の1つであるIESSと乳児健常群で有意差は認められなかった。日本人の腸内細菌叢は欧州よりで、中国とは異なることが報告されているが、今回本研究で有意差は認められなかったのは民族の違いによるかどうかは今後症例を蓄積して検討していく予定である。