2022 Fiscal Year Final Research Report
Elucidation of appropriate excersice protocol through AMPK/OGT crosstalk in T2B
| Project/Area Number |
20K11352
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Review Section |
Basic Section 59020:Sports sciences-related
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| Research Institution | Osaka Medical and Pharmaceutical University |
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Principal Investigator |
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| Project Period (FY) |
2020-04-01 – 2023-03-31
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| Keywords | Exercise / オートファジー |
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| Outline of Final Research Achievements |
Exercise is one of the most important therapies for diabetes, especially type 2 diabetes, T2B. Appropriate exercise decreases blood sugar through activation of AMP-activated kinase (AMPK) in an insulin independent manner. On the other hand, increased protein O-β-N-acetylglucosamine (O-GlcNAcylation) caused by high blood sugar aggravates pathogenesis of T2B. The aim of the study was to gain insight into the role of AMPK/OGT crosstalk in exercise therapy of T2B and find a way to maximize it. Achievements of the research are: 1. Establishment of in vitro assay of membrane translocation of Glut 4 using mouse myoblast cell line, C2C12 cells, by evaluating the effect of exercise with treadmill using mice on AMPK/OGT crosstalk and autophagy, then 2. Identification of autophagy in this system, ３．Induction of autophagy by appropriate exercise with treadmill using mice, which was cancelled with Thiamet G, OGA inhibitor.
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| Free Research Field |
薬理学、生化学
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| Academic Significance and Societal Importance of the Research Achievements |
運動療法は、中心的なT2B治療である。今回、細胞の栄養センサーと呼ばれるAMPKと高血糖から引き起こされるタンパク質O-GlcNAc との関連、AMPK/O-GlcNAcクロストークの一端をin vivo運動負荷モデルにおいて観察できたことは、学術的に有意義なことである。さらなる研究が必要ではあるが、AMPK/O-GlcNAcクロストークを人工的に制御することが出来れば、現在、1000万人弱の糖尿病患者（予備群にを含めるとその倍以上いると考えられる）にとって朗報となるのみならず、社会保険的意義も非常に高いと考えられる。
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