2021 Fiscal Year Research-status Report
腸におけるGPCRを介したアディポネクチン発現制御機構の解明と抗糖尿病効果の検討
| Project/Area Number |
20K11532
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| Research Institution | 株式会社レオロジー機能食品研究所 |
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Principal Investigator |
Hossain Shamim 株式会社レオロジー機能食品研究所, 未登録, 基礎研究部長 (40647707)
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| Project Period (FY) |
2020-04-01 – 2023-03-31
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| Keywords | plasmalogens / GPR61 / Adiponectin / Lipid metabolism / Intestinal cells |
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| Outline of Annual Research Achievements |
The role of GPR61 in the regulation of Adiponectin expression was confirmed in the intestinal cells by establishing general GPR61 knockout mice as the knockout mice had less adiponectin in the intestinal cells compared to the wildtype mice. Plasmalogens (Pls)-mediated increase of Adiponectin expression in the intestinal epithelial cells of adult mice has been confirmed by primary culture of the intestinal cells. Oral ingestion of plasmalogens reduced the fatty liver in the ZDF rats associated with the increase of Adiponectin in blood serum. We also checked various gene expression related to fatty acid biosynthesis and metabolism in the liver tissue of ZDF rat and found some candidate genes of plasmalogens,which could be the mechanism of Pls-mediated reduction of the fatty liver.
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| Current Status of Research Progress |
Current Status of Research Progress
2: Research has progressed on the whole more than it was originally planned.
Reason
Some part of the projects are progressed very well and I could also successfully make generals GPR61 knockout mice to support my research findings.
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| Strategy for Future Research Activity |
By using the general knockout mice of GPR61,I well study the role of plasmalogens in the regulation of Adiponectin and fatty acid matabolism in the liver. I will study the detail lipid biosynthesis pathway to find out the mechanism of how plasmalogens can reduce fatty liver.
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