2022 Fiscal Year Final Research Report
Creation of peptide-silica adsorption carriers with selective metal recognition ability imprinted with target metal
| Project/Area Number |
20K12232
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Review Section |
Basic Section 64030:Environmental materials and recycle technology-related
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| Research Institution | Akita University |
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Principal Investigator |
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| Project Period (FY) |
2020-04-01 – 2023-03-31
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| Keywords | 分子インプリント / バイオシリカ / ペプチド / 金属回収 |
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| Outline of Final Research Achievements |
This study is a basic investigation aimed at developing selective metal adsorption carriers with molecular imprinting of targeted metals with peptides and silica through the use of biological functions. R5H6 was designed as a peptide to be used, which consists of a silica-forming peptide (R5) from diatom and six histidine residues with metal-coordination ability, and a recombinant microorganisms carrying the cDNA were constructed to examine R5H6 production and silica-forming activity. Furthermore, to promote the growth of silica crystals, we constructed a recombinant E. coli expressing the silica-forming enzyme silicatein and demonstrated that it can be mass-produced by refolding operation.
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| Free Research Field |
生物化学工学
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| Academic Significance and Societal Importance of the Research Achievements |
本研究は生体機能および生体材料に着想を得てこれを模倣・利用し，ペプチドとシリカを融合させる全く新しい手法により選択的な分子インプリント担体のグリーン合成を目指す点に本研究の特色がある。特にシリカ粒子への分子インプリントは，環境にやさしい穏和な条件でシリカ結晶生成を誘起するペプチドやシリカ重合酵素を利用するものとして，従来法とは異なる独創的なものである。さらに，標的分子の吸脱着も中性pH領域で行われることから，環境にやさしい分離プロセスの構築も期待できる。本研究は，その基礎となるペプチドおよびシリカ重合酵素の遺伝子組換え微生物による大量合成の可能性を示したものである。
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