Studies on the cluster-ion-specific mutagenesis and industrial application

2022 Fiscal Year Final Research Report

• Project/Area Number: 20K12489
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 80040:Quantum beam science-related
• Research Institution: National Institutes for Quantum Science and Technology
• Principal Investigator: Hase Yoshihiro 国立研究開発法人量子科学技術研究開発機構, 高崎量子応用研究所 放射線生物応用研究部, 上席研究員 (70354959)
• Co-Investigator(Kenkyū-buntansha): 森林 健悟 国立研究開発法人量子科学技術研究開発機構, 量子生命科学領域, 上席研究員(定常) (70354975)
• Project Period (FY): 2020-04-01 – 2023-03-31
• Keywords: クラスターイオン / 枯草菌 / 致死効果 / 突然変異

Outline of Final Research Achievements

We examined the biological effects of cluster ion beams, which has never been used for the irradiation of biological samples, by using the spores of Bacillus subtilis as a model organism. Mono-layered spore samples prepared on silicon wafer were irradiated in a vacuum chamber and the survival rate was determined. The 2 MeV H2+ showed a lower lethality than 340 MeV H+, although both have a comparable linear energy transfer. The estimated internuclear distance of two protons of 2 MeV H2+ and the radial dose distribution suggest that the spread of deposited total energy over two areas separated by just several nm greatly diminishes the lethality and that the energy density in the very center of the trajectory, possibility within a 1 nm radius, has a great impact on the lethality.

Free Research Field

量子ビーム科学

Academic Significance and Societal Importance of the Research Achievements

本研究では、クラスターイオン特有のエネルギー付与を利用することによって、軌道中心のエネルギー密度が致死効果に大きく寄与することを初めて実験的に示唆する結果を得た。高LET放射線の生物効果を理解するツールの1つとして、クラスターイオンの活用が期待される。