2022 Fiscal Year Final Research Report
Ex vivo reconstruction of hematopoietic function using 3D culture of umbilical cord blood cells
| Project/Area Number |
20K12592
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Review Section |
Basic Section 90110:Biomedical engineering-related
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| Research Institution | University of Tsukuba |
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Principal Investigator |
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| Project Period (FY) |
2020-04-01 – 2023-03-31
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| Keywords | 造血幹細胞 / ストローマ細胞 / 三次元培養 / 共培養 / 臍帯血 / PVA / ティッシュ・エンジニアリング |
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| Outline of Final Research Achievements |
We investigated ex vivo expansion of hematopoietic stem cells (HSCs) derived from umbilical cord blood (UCB) in a three-dimensional (3D) culture system for application to UCB transplantation. In this study, the effects of autologous cord blood-derived materials on the expansion of HSCs were examined.
As autologous materials, stromal cells and UCB-plasma both contained in UCB were chosen. We found that addition of UCB-plasma facilitated expansion of HSCs, whereas application of stromal cells was difficult due to scarce proliferation ability of the cells. Since the amount of plasma in UCB is limited, we next investigated the possibility of reducing the amount of cord blood plasma by adding polyvinyl alcohol (PVA) to the culture medium. From the 3D cultures under different cell density and culture medium conditions, PVA was found to be effective under conditions where expansion of HSCs was difficult.
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| Free Research Field |
再生医工学
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| Academic Significance and Societal Importance of the Research Achievements |
造血幹細胞移植の中で、臍帯血移植は一般的な骨髄移植に比べて細胞の採取が容易であり、ヒト白血球抗原のミスマッチに対して寛容であるという利点がある。しかし、採取できる造血幹細胞数が少ないため、成人への移植に応用するためには培養系において造血幹細胞を増幅する必要がある。この際、できるだけ安全性の高い方法で増幅する必要があることから、本研究では自己臍帯血由来の材料を用いて増幅を試みたところ、臍帯血血漿が有効であることが確かめられた。同成分は臍帯血の調製過程で廃棄される成分であることから、本研究の結果は、低コストかつ安全に造血幹細胞を増幅できる手法の確立に貢献すると期待される。
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