2022 Fiscal Year Final Research Report
Integrated organoid system with mature vascular network and long-term perfusion
| Project/Area Number |
20K12608
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Review Section |
Basic Section 90110:Biomedical engineering-related
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| Research Institution | Shibaura Institute of Technology |
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Principal Investigator |
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| Project Period (FY) |
2020-04-01 – 2023-03-31
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| Keywords | 血管新生 / マイクロ流体 / リモデリング / 長期灌流培養 |
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| Outline of Final Research Achievements |
Vascular endothelial cells form capillary structures through a long-term process of angiogenesis and remodeling. Reproducing such processes in vitro will contribute to regenerative medicine, especially the artificial construction of transplantable thick tissues.
We have produced mature lumen structures in vitro by combining a 3D culture of vascular endothelial cells in fibrin gel and a Braille-based microfluidic perfusion device that dynamically changes perfusion directions and flow rates. In addition, time evolution data of these lumen structures provided experimental evidence of the relationship between flow and angiogenesis.
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| Free Research Field |
生体医工学
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| Academic Significance and Societal Importance of the Research Achievements |
完全に自発的な血管網をin vitroで再現．組織形成途上の細胞の状態に依らないオルガノイド形成技術を確立できれば，オルガノイドの大型化に貢献できる． また，オンチップセルプロセシングシステムとしての応用．細胞密度や周囲の環境に適応できる自動培養システムへの応用が期待できる．さらに，集積オルガノイド・プロセシングシステムへの発展．毛細血管網オルガノイドにコンパクトな循環灌流系と雰囲気制御系をセットにして，（オルガノイド＋長期潅流培養＋アッセイ）を一体化することで，薬剤の長期的な影響の評価への対応が容易となる．
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