Elucidation of the role of miRNA on the control of cellular function by ultrasound

2022 Fiscal Year Final Research Report

• Project/Area Number: 20K12619
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 90110:Biomedical engineering-related
• Research Institution: University of Toyama
• Principal Investigator: Tabuchi Yoshiaki 富山大学, 学術研究部薬学・和漢系, 教授 (20322109)
• Co-Investigator(Kenkyū-buntansha): 長谷川 英之 富山大学, 学術研究部工学系, 教授 (00344698) ; 鈴木 信雄 金沢大学, 環日本海域環境研究センター, 教授 (60242476) ; 古澤 之裕 富山県立大学, 工学部, 准教授 (80632306) ; 平山 順 公立小松大学, 保健医療学部, 教授 (90510363)
• Project Period (FY): 2020-04-01 – 2023-03-31
• Keywords: 超音波 / 細胞分化 / アクチン

Outline of Final Research Achievements

In order to elucidate the cellular response to low-intensity pulsed ultrasound (LIPUS), the effects of LIPUS on the osteogenic differentiation in osteoblastic cells were investigated. Mouse osteoblastic MC3T3-E1 cells were exposed to LIPUS (30 mW/cm2 for 20 min; once a day). Repeated exposure of LIPUS significantly elevated mineralization level detected using alizarin red staining. Morphological changes were observed in the cells treated with siRNA for actin beta. The silencing of actin beta diminished the osteoblastic differentiation that was increased in the MC3T3-E1 cells by exposure to LIPUS. These findings will provide a molecular basis for further understanding of the mechanisms of LIPUS in osteoblastic differentiation.

Free Research Field

細胞生理学

Academic Significance and Societal Importance of the Research Achievements

低出力パルス超音波 (LIPUS) は，骨折治癒促進効果があるがその分子機構は完全に解明されていない．LIPUSの細胞応答を明らかにするために，骨芽細胞の骨分化に対するLIPUSの効果を検討した．マウス骨芽MC3T3-E1細胞へのLIPUSの連続照射は，骨分化を有意に上昇させ，この作用の少なくとも一部にアクチンベータが関与することが判った．得られた成績は， LIPUSの作用メカニズムを理解するための重要な分子基盤になる．