2023 Fiscal Year Annual Research Report
Novel label-free tool for infections diagnosis based on Nano-Electro Optical Tweezers
| Project/Area Number |
20K12701
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| Research Institution | Okinawa Institute of Science and Technology Graduate University |
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Principal Investigator |
KOTSIFAKI Domna 沖縄科学技術大学院大学, 量子技術のための光・物質相互作用ユニット, 客員研究員 (70834117)
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| Project Period (FY) |
2020-04-01 – 2024-03-31
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| Keywords | Raman Spectroscopy / Nano-apertures / Metallic devices / Bacteria |
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| Outline of Annual Research Achievements |
By employing the unique electromagnetic properties of metamaterials, this year I focused on integrating Fano-resonant metamolecules to enhance Raman scattering signals from bacterial components. By exploiting off-resonance laser excitation and local immobilization of microorganisms on metamaterial surfaces, I have overcome existing challenges associated with weak Raman signals and photodamage effects, enabling robust detection even in complex liquid matrices. This part of the project involved the design, fabrication, and optimization of Fano-enhanced Raman scattering platforms tailored for microbial detection. Experimental studies were conducted using Escherichia coli as a model microorganism, with Raman signatures recorded at various growth phases. I have cultured two different solutions with bacteria in mid-exponential phase and stationary phase. The time-dependent FERS signal analyzed to understand the dynamics of bacterial biochemical composition and its correlation with growth stages. Additionally, the platform's performance was validated using diverse microbial strains and real-world liquid samples to assess its suitability for large-scale applications. The results of this study have been published in Biomedical Optics Express.
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Research Products
(1 results)
