Study of Generalized Conditions in Protein Direct Determination on Fiber Substrates

2022 Fiscal Year Final Research Report

• Project/Area Number: 20K13808
• Research Category: Grant-in-Aid for Early-Career Scientists
• Allocation Type: Multi-year Fund
• Review Section: Basic Section 08030:Family and consumer sciences, and culture and living-related
• Research Institution: Jissen Women's University
• Principal Investigator: Tsukazaki Mai 実践女子大学, 生活科学部, 助教 (50844924)
• Project Period (FY): 2020-04-01 – 2023-03-31
• Keywords: BCA法 / タンパク質汚れ / 定量 / 布 / 皮脂 / 界面活性剤 / 繊維 / 反射率

Outline of Final Research Achievements

This study aimed to develop novel method for directly determining proteins adhering to fabric by measuring the reflectance of the BCA method color reaction. It also investigated the effect of the type of protein and the fabric used as the substrate; as possible factors influencing this determination method. Additionally, for application to cleaning research, it examined the effect of surfactants and enzymes contained in laundry detergents; and fatty acids compounded as sebum soil which coexist in this determination method.
As a result, a good correlation was obtained between the amount of protein on the fabric and the K/S value, indicating that direct quantification of protein is possible as a calibration curve. Although affected to some extent by specific amino acid groups, moisture retention of the fabric, and other factors, it was found to be less affected by surfactants, and have highly versatility this quantification method.

Free Research Field

被服整理学、染色化学

Academic Significance and Societal Importance of the Research Achievements

本研究により、これまで検討されていない、布上の無色のタンパク質を高感度で簡便に直接定量する方法を明らかにし、洗浄研究への適用に向けた基礎的な知見を得ることができた。今後、さらに実際の汚れに近い条件の検討を進めていくことで、実験者の技量や、複雑で時間を要する機器・手順を必要とせず、数多くの試料を扱うことが容易となり、例えば、タンパク質汚れに特化した新規洗浄剤、防汚素材の開発を目指す、幅広い分野の研究者にとって、スピーディで精度の良い定量分析を行う手段として貢献できると考える。また、視覚的に被服の汚れを捉えやすく、色相でタンパク質汚れ量を推定する教材としての活用も期待できると考える。