2020 Fiscal Year Research-status Report
Elucidating plasma effect on structural modification of antioxidant enzymes: Combined experimental and computational
| Project/Area Number |
20K14454
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| Research Institution | Kyushu University |
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Principal Investigator |
Attri Pankaj 九州大学, プラズマナノ界面工学センター, 准教授 (40868361)
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| Project Period (FY) |
2020-04-01 – 2023-03-31
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| Keywords | Computational study / SARS-CoV-2 / NADPH oxidase / Biophysical techniques / Plasma treated liquid / MDM2-p53 complex |
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| Outline of Annual Research Achievements |
Project 1: Structural modification of NADPH oxidase activator (Noxa 1) by oxidative stress: An experimental and computational study; NADPH oxidases 1 (NOX1) derived reactive oxygen species (ROS) play an important role in the progression of cancer through signaling pathways. Therefore, in this paper, we demonstrate the effect of cold atmospheric plasma (CAP) on the structural changes of Noxa1 SH3 protein, one of the regulatory subunits of NOX1. The structural deformation of Noxa1 SH3 protein was analyzed by various experimental methods and by MD simulations. Publication: Pankaj Attri*, et al, International Journal of Biological Macromolecules 2020, 163, 2405-2414. IF = 5.16
Project 2: Possible impact of plasma oxidation on the structure of C-terminal Domain of SARS-CoV-2 spike protein: A computational study. Cold atmospheric plasma (CAP) has shown its potential application in sterilization. We check the CAP's possible effect on the structure of the C-terminal domain of SARS-CoV-2 (SARS-CoV-2-CTD) spike protein and interaction SARS-CoV-2-CTD with human Angiotensin-Converting Enzyme 2 (hACE2). Publication: Pankaj Attri*, et al, Applied Physics Express 2021, 14, 027002. IF = 3.01
Project 3: Impact of seed color and storage time on the radish seed germination and sprout growth in plasma agriculture. This study reveals that Air scalar-DBD plasma's reactive oxygen and nitrogen species (RONS) can efficiently accelerate germination and growth in older seeds. Publication: Pankaj Attri*, et al, Scientific Reports 2021, 11, 2539. IF = 3.99
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| Current Status of Research Progress |
Current Status of Research Progress
3: Progress in research has been slightly delayed.
Reason
Due to the COVID19, some experiments are detailed due to restriction in University and unable to access the machines used for analysis.
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| Strategy for Future Research Activity |
Ongoing Project 1: Impact of plasma oxidation on MDM2-p53 complex. Protein-protein interaction study is of great interest. Several early studies focused on the murine double minute 2 (Mdm2)- tumor suppressor protein p53 interactions. In this study, we will investigate the structural changes in Mdm2, p53, and Mdm2-p53 complex before and after possible plasma oxidation through molecular dynamic (MD) simulations. Later, we will explore the anticancer activity and change in expression of Mdm2 and p53.
Ongoing Project 2: Plasma treatment causes structural modifications in lysozyme, with possible applications for cancer treatment. This study aims to modify the lysozyme structure using cold atmospheric plasma to boost its cancer cell killing effect. We investigated the structure at acidic and neutral pH using various experimental techniques and molecular dynamics simulations. The plasma-treated lysozyme was found to selectively kill cancer cells and without damaging the normal cells normal cells at low concentrations.
Ongoing Project 3: Green route for ammonium nitrate synthesis: Fertilizer for plant growth enhancement. This study aims to synthesize the N-enriched plant soil (NH4NO3 fertilizer in soil) using the low-temperature and low-pressure plasma [without H2 and external catalyst]. Subsequently, we used plasma N- enriched soil for plants (radish and tomato) growth. The energy consumption (EC) for NH4NO3 fixation was 12 MJ/mol. Further to understand the plasma chemistry, we performed 1D simulation using COMSOL Multiphysics software. T
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[Journal Article] Structural modification of NADPH oxidase activator (Noxa 1) by oxidative stress: An experimental and computational study2020
Author(s)
Pankaj Attri*, J.-H. Park, J. D. Backer, M. Kim, J.-H. Yun, Y. Heo, S. Dewilde, M. Shiratani, E. H. Choi, W. Lee, A. Bogaerts
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Journal Title
International Journal of Biological Macromolecules
Volume: 163
Pages: 2405-2414
DOI
Peer Reviewed / Int'l Joint Research
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