2021 Fiscal Year Final Research Report
Experimental study on molecular dynamics of water to design greener cryopreservation process of cells
| Project/Area Number |
20K14662
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| Research Category |
Grant-in-Aid for Early-Career Scientists
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| Allocation Type | Multi-year Fund |
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| Review Section |
Basic Section 19020:Thermal engineering-related
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| Research Institution | The University of Tokyo |
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Principal Investigator |
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| Project Period (FY) |
2020-04-01 – 2022-03-31
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| Keywords | バイオ熱工学 / 誘電分光 / 水分子ダイナミクス / 細胞凍結保存 / 凍結保護物質 |
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| Outline of Final Research Achievements |
In this study, the molecular dynamics of water in cells was investigated by the dielectric spectroscopy, with the aim to have a better knowledge about how the intracellular water molecules behave with the existence of cryoprotective agents used in cryopreservation of cells. An experimental setup for the dielectric spectroscopy of cell suspensions was built to obtain dielectric spectra for the frequency range from 40 Hz to 43.5 GHz. Using this setup, measurements were performed on Jurkat cells suspended in sucrose aqueous solutions of different concentrations at a temperature of 25 °C to evaluate the effect of cell dehydration. By the analysis of dielectric spectra, the difference in the distribution of molecular dynamics of water due to the dehydration was shown. In addition, dielectric spectra for cells suspended in cryoprotective solutions were obtained at the temperature range of 10 °C to -39 °C, which leads to understanding of the water behavior in cryopreservation processes.
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| Free Research Field |
熱工学
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| Academic Significance and Societal Importance of the Research Achievements |
本研究で着目した細胞内の水分子ダイナミクスは, 細胞内の氷の核生成頻度に関連していると考えられ, その実験的な評価手法の整備により, 様々な現象が関わる複雑な細胞凍結保存プロセスについての理解につながりうるという学術的意義に加えて, 細胞内の水分子ダイナミクスを指針の一つとして細胞凍結保存プロセスの効率化が実現されれば, 細胞の凍結保存が不可欠な再生医療や免疫療法, 不妊治療等の拡がりに寄与しうるという社会的意義を有する.
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