2022 Fiscal Year Final Research Report
High pressure study on a living cell using total reflection spectroscopy
| Project/Area Number |
20K15225
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| Research Category |
Grant-in-Aid for Early-Career Scientists
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| Allocation Type | Multi-year Fund |
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| Review Section |
Basic Section 32010:Fundamental physical chemistry-related
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| Research Institution | Kobe Gakuin University |
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Principal Investigator |
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| Project Period (FY) |
2020-04-01 – 2023-03-31
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| Keywords | 高圧 / 分光 / 細胞膜 |
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| Outline of Final Research Achievements |
In order to elucidate the survival mechanism of deep-sea organisms, in-situ measurements of the behavior of single cell under high-pressure will provide a useful clues. In this study, we developed a high-pressure optical cell applicable to total-reflection microscopic raman or fluorescence spectroscopies. As a preliminary experiments, using liposomes which is often used as a model for cell membrabe, we showed that pressure effects on vibrational spectrum of lipid bilayers can actually provide information on its phase transition by pressure. Furthermore, we succeeded in culturing cells on a high-pressure cell and in observing them under high-pressure.The morphology of cells seemed not to be altered by pressure. Although we couldn't complete the project during the funding period, we established the basis for further in-situ high-pressure measurements.
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| Free Research Field |
生物物理化学
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| Academic Significance and Societal Importance of the Research Achievements |
深海生物の高圧下での生存戦略を化学の視点から明らかにすることで、極限環境にも適用可能な有用な酵素や微生物などの開発につながることが期待される。そのために、本研究は生細胞の圧力挙動を明らかにすることを目指し、圧力下での測定1細胞の測定が可能な特殊高圧セルの開発と高圧セルを用いた予備的実験を行った。本研究成果は、極限環境生物を理解する上で必要となる生細胞の圧力下その場測定の基礎になると考えられる、
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