Super-sensitive Raman spectroscopy by fluorescence encoding

2022 Fiscal Year Final Research Report

• Project/Area Number: 20K15227
• Research Category: Grant-in-Aid for Early-Career Scientists
• Allocation Type: Multi-year Fund
• Review Section: Basic Section 32010:Fundamental physical chemistry-related
• Research Institution: The University of Tokyo
• Principal Investigator: Hiramatsu Kotaro 東京大学, 大学院理学系研究科(理学部), 助教 (60783561)
• Project Period (FY): 2020-04-01 – 2023-03-31
• Keywords: 蛍光エンコーディング / ラマン分光法 / 振動分光法 / 蛍光

Outline of Final Research Achievements

Fluorescence-encoded time-domain coherent Raman spectroscopy (FLETCHERS) was developed by combining time-domain Raman spectroscopy and fluorescence encoding to achieve highly sensitive Raman spectroscopy in the low-frequency region (200-750 cm-1). FLETCHERS is a time-domain coherent Raman spectroscopy technique that uses a single ultrashort pulse of light to excite molecular vibrations and measure the temporal evolution of the vibrations by changing the intensity of the fluorescence measured by another ultrashort pulse of light. We have demonstrated that it is possible to measure the vibrational spectrum of Rhodamine 800 in a solution with a concentration of 250 nM.

Free Research Field

分子分光学

Academic Significance and Societal Importance of the Research Achievements

ラマン分光法は化学分析やバイオイメージングにおける計測モダリティとして広く用いられている．ラマン分光は無標識に分子構造情報を得られる強力な手法である一方で，その感度の低さから応用範囲が限定されている．本研究で開発したFLETCHERSは蛍光分子の振動スペクトルを高感度に検出でき，バイオイメージングの多色化，材料中の欠損の評価，1分子レベルでの環境ゆらぎの検出などへの応用が期待される．