2021 Fiscal Year Final Research Report
Development and in vivo application of hyperpolarized molecular probes for aminopeptidase activities
Project/Area Number |
20K15396
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Research Category |
Grant-in-Aid for Early-Career Scientists
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Allocation Type | Multi-year Fund |
Review Section |
Basic Section 37010:Bio-related chemistry
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Research Institution | The University of Tokyo |
Principal Investigator |
Saito Yutaro 東京大学, 大学院工学系研究科(工学部), 助教 (10846281)
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Project Period (FY) |
2020-04-01 – 2022-03-31
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Keywords | 超核偏極 / MRI / アミノペプチダーゼ / 分子プローブ |
Outline of Final Research Achievements |
Dynamic nuclear polarization (DNP) is a technique that dramatically enhances MRI sensitivity and is expected to be applied to next-generation diagnostic techniques. On the other hand, many issues remain in the development of molecular probes necessary for the application of this technology. In particular, the lack of a wide variety of molecular probes applicable in vivo and the absence of guidelines for designing new molecular probes have been major problems. In this study, it was attempted to develop DNP-MRI molecular probes that detect the activity of aminopeptidases, a group of enzymes that are important in biological phenomena. As a result, a new DNP-MRI molecular probe was developed by precisely designing the molecule targeting aminopeptidase N (APN), which is closely related to angiogenesis, metastasis, and malignancy of cancer. The developed probe was applied to animal models and succeeded in detecting APN activity in vivo and visualizing enzyme activity in tumors.
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Free Research Field |
ケミカルバイオロジー
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Academic Significance and Societal Importance of the Research Achievements |
動的核偏極 (DNP) 法を用いた核磁気共鳴イメージング (MRI) は、新しい早期診断法や疾患メカニズム解明につながる技術として注目されている。一方、この技術を医療や生命科学に応用するには課題が残されている。本研究成果は、その課題の一つである、分子プローブの多様性の欠乏を解決することに貢献できるものと考えられる。今後、この成果によって得られた分子設計指針を活用することでさらに新しい分子プローブが開発されることが期待される。
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