2021 Fiscal Year Final Research Report
Photo-ligation of SNA via photo-responsive nucleobases
| Project/Area Number |
20K15399
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| Research Category |
Grant-in-Aid for Early-Career Scientists
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| Allocation Type | Multi-year Fund |
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| Review Section |
Basic Section 37010:Bio-related chemistry
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| Research Institution | Nagoya University |
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Principal Investigator |
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| Project Period (FY) |
2020-04-01 – 2022-03-31
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| Keywords | SNA / 光架橋 / ライゲーション / RNA / 人工核酸 |
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| Outline of Final Research Achievements |
Ligation of nucleic acids generates complementary strand for template from fragment strands via formation of phosphodiester bond, which is significantly important for various biological technology including genetic recombination, nucleic acid architecture, and nucleic acid detection. In this project, we have newly developed photo-ligation of SNA, artificial nucleic acid, on a template RNA strand. SNA fragments carrying photo-responsive nucleobase can be effectively ligated on RNA template by the irradiation of 365 nm light.
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| Free Research Field |
生物有機化学
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| Academic Significance and Societal Importance of the Research Achievements |
今回開発した光ライゲーション反応は特定のRNA配列存在下でのみSNA鎖を連結する反応であるため、RNAを標的とする新たなRNA検出システムや核酸医薬としての応用が期待できる。また、本研究の過程で新たに開発した光応答性核酸塩基NVAは、過去に報告しているPVAと同様の二重鎖光制御を、より短波長の光照射で行うことができるため、PVAとNVAを併用することで複雑な核酸分子マシンを構築することが可能となった。
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