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2021 Fiscal Year Final Research Report

Molecular mechanism of action of JA analogue which selectively enhances antiherbivore defense in Solanaceae plants

Research Project

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Project/Area Number 20K15404
Research Category

Grant-in-Aid for Early-Career Scientists

Allocation TypeMulti-year Fund
Review Section Basic Section 37020:Chemistry and chemical methodology of biomolecules-related
Research InstitutionTohoku University

Principal Investigator

Kaji Takuya  東北大学, 理学研究科, 助教 (80835520)

Project Period (FY) 2020-04-01 – 2022-03-31
Keywordsケミカルバイオロジー / 植物ホルモン / タンパク-タンパク間相互作用
Outline of Final Research Achievements

Plant hormone JA-Ile induces defense response against insects and pathogens, but also induces growth inhibition. Jasmonoyl-L-isoleucine lacton (JILa), which is a synthetic lactone analogue of JA-Ile, was reported to show defense response without causing growth inhibition against tobacco. However, detailed mechanism of action remained elucive. In this study, we conducted bioactivity measurements and in-vitro biochemical mechanistic analysis using tomato, the same Solanaceae model plant as tobacco. Our results suggested that JILa induced tomatine accumulation, and the bioactive form of JILa was suggested as its hydrolysate, 12-OH-JA-Ile. In-vitro assay and gene expression analysis suggested that 12-OH-JA-Ile would selectively activate partial jasmonate signaling through receptor subtype selective binding activity.

Free Research Field

ケミカルバイオロジー

Academic Significance and Societal Importance of the Research Achievements

植物において外敵への防御応答は様々な方法で制御されている。ジャスモン酸による防御応答については生長と防御のトレードオフを伴うことが知られていた。本研究では、このトレードオフの切り分けを生じるジャスモン酸類縁体に着目し、生物活性が報告されたタバコと同じナス科植物トマトでの作用機構解明に取り組んだ。トマトに着目したのは有用な商品作物であり、生物活性を有する特化代謝物トマチンを生合成すること、またジャスモン酸関連の変異体や遺伝子資源が利用できるためである。得られた知見はジャスモン酸応答の一部活性化に関する新しいメカニズムの一端を示唆するものであり、ジャスモン酸の人為的な活用に有用な知見である。

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Published: 2023-01-30  

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