2022 Fiscal Year Final Research Report
Development of Raman tags to detect the small biomolecules
| Project/Area Number |
20K15418
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| Research Category |
Grant-in-Aid for Early-Career Scientists
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| Allocation Type | Multi-year Fund |
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| Review Section |
Basic Section 37030:Chemical biology-related
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| Research Institution | Institute of Physical and Chemical Research |
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Principal Investigator |
Egoshi Syusuke 国立研究開発法人理化学研究所, 開拓研究本部, 研究員 (60755932)
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| Project Period (FY) |
2020-04-01 – 2023-03-31
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| Keywords | ラマンイメージング / アルキン / 安定同位体元素 |
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| Outline of Final Research Achievements |
We found that vibrational frequencies of terminal alkyne were shifted 135 cm-1 upon deuteration. By using the protonated and deuterated alkynes, we achieved the two-color Raman imaging to monitor the intracellular distributions of similar small biomolecules simultaneously, such as a stearic acid and a oleic acid. This imaging technique can be adapted to detect various small molecules in living cells and is expected to be applied various research fields.
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| Free Research Field |
ケミカルバイオロジー
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| Academic Significance and Societal Importance of the Research Achievements |
本研究で開発されたイメージング法を用いることで，構造の類似する複数の低分子化合物間の相互作用を観察することが可能となった．また，本手法は低分子化合物に限らず様々な生体内分子にも適応可能であり，複雑な生体内分子間の相互作用の解析へ応用展開されることが期待される．
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