2022 Fiscal Year Final Research Report
Establishment of a general heterologous expression system for difficult-to-express molybdenum enzymes and their functional analysis.
Project/Area Number |
20K15432
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Research Category |
Grant-in-Aid for Early-Career Scientists
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Allocation Type | Multi-year Fund |
Review Section |
Basic Section 38020:Applied microbiology-related
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Research Institution | Kyoto University |
Principal Investigator |
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Project Period (FY) |
2020-04-01 – 2023-03-31
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Keywords | モリブデン酵素 |
Outline of Final Research Achievements |
Molybdenum enzymes are unique redox enzymes that catalyze a variety of reactions. By using Rhodococcus erythropolis as a heterologous expression, I succeeded in heterologous (partially homologous) expression of uracil/thymine dehydrogenase, xanthine oxidase, and polyphenol dehydroxylase. After examining the conditions for heterologous expression in the active form, I found that the addition of molybdic acid and optimization of codon frequency were important for heterologous expression in the active form.
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Free Research Field |
応用微生物学
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Academic Significance and Societal Importance of the Research Achievements |
モリブデン酵素は、様々な反応を触媒するユニークな酸化還元酵素であり、その反応の多 様性から、幅広い分野に応用可能な酵素である。例えば、ギ酸脱水素酵素による二酸化炭素が還元や、腸内細菌のポリフェノール脱水素酵素による腸内代謝など、環境分野や健康分野といった様々な分野に関与する重要な酵素である。モリブデン酵素の活性型での異種発現に関する知見は、モリブデン酵素の学術的知見を深めるうえで必要不可欠であり、その応用は幅広い分野に役立ち、社会的意義が高いものである。
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