2023 Fiscal Year Final Research Report
Regulation of bacterial proteostasis by novel enzyme reactions: a strategy for adaptation to oxidative stress that is alien to us.
| Project/Area Number |
20K15446
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| Research Category |
Grant-in-Aid for Early-Career Scientists
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| Allocation Type | Multi-year Fund |
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| Review Section |
Basic Section 38020:Applied microbiology-related
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| Research Institution | Hyogo Prefectural Institute of Technology |
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Principal Investigator |
Imai Takeshi 兵庫県立工業技術センター, その他部局等, 主任研究員 (30785241)
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| Project Period (FY) |
2020-04-01 – 2024-03-31
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| Keywords | 過酸化タンパク質 / 細菌 / 抗生剤 / trHbO / 切断型ヘモグロビン / バイオフィルム |
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| Outline of Final Research Achievements |
This research theme has revealed that trHbO, a protein of unknown function that is widely conserved in bacterial species, has the ability to reduce peroxidised proteins. In particular, it was found that trHbO inhibits the oxidative denaturation and aggregation of proteins that are in frequent contact with atmospheric oxygen, such as TasA, which constitutes biofilms, and actually prevents cell damage at the level of physiological functions. It was also suggested that protein peroxidation is also induced by typical oxidising agents such as hypochlorous acid, indicating that trHbO, which is responsible for the reduction of peroxidised proteins, is a potential target for the development of new antibiotics.
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| Free Research Field |
分子微生物学
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| Academic Significance and Societal Importance of the Research Achievements |
グラム陽性菌である結核菌に有効な抗生剤は種類が限られており、とりわけスーパーバグと呼ばれるような超多剤耐性菌の出現も確認されている．trHbOはグラム陽性菌に広く保存ざれている機能不明なタンパク質であったが、今回の研究によって強力な抗酸化の機能を持つことが明らかとなった．将来的には、結核菌などの病原性細菌中のtrHbOの働きを阻害することで、自然免疫のような酸化ストレスに対して脆弱化させる、新しいコンセプトの抗生剤開発が期待される．
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