2020 Fiscal Year Research-status Report
The potential beneficial effects of inactivated Bifidobacterium Longum on the alleviation of muscle atrophy and physical fatigue in aged hospitalized and athletes using in vitro and in vivo models.
| Project/Area Number |
20K15466
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| Research Institution | University of Tsukuba |
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Principal Investigator |
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| Project Period (FY) |
2020-04-01 – 2023-03-31
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| Keywords | Fatigue / Treadmill / FST / Power strength / Lactic acid / ATP / Glycogen |
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| Outline of Annual Research Achievements |
As planned for this fiscal year, we investigated the effect of IBL on fatigue in C57BL/6J mice. In order to induce fatigue, mice were exposed to two different exercises; forced swimming test (FST) and running test (treadmill). Mice were exposed to two different exercises; forced swimming test (FST) and running test (treadmill). After five weeks of treatment, mice were exposed to both exercises and results were recorded. Muscles and livers were weighing and keeping for ATP and glycogen measurements. In addition, serum samples were preserved for blood urea nitrogen (BUN) and lactic acid (LA) analysis.
FST results showed a significant increase of mobility time in 50 mg/kg and 100 mg/kg groups to reach 360 s and 440 s, respectively, compared with positive control (200 s). Moreover, the results of running test showed a significant effect of IBL at 50 mg/kg (1100 m) and 100 mg/kg (1300 m) on running distance of mice in dose dependent manner. After both exercises, the power strength of mice muscles was measured. Results showed a significant decrease of muscles strength in the non-treated control group compared with non-fatigue normal control. Furthermore, the power strength of treated mice was significantly increased.
Blood serum analysis showed that IBL significantly increased BUN compared with positive control group. in addition, IBL significantly reduced LA which is the main reason of fatigue feeling. As well, glycogen in muscles and liver was measured. Muscles and liver analysis showed a significant decrease of glycogen levels in IBL-treated mice.
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| Current Status of Research Progress |
Current Status of Research Progress
2: Research has progressed on the whole more than it was originally planned.
Reason
The research progress was going well and as expected, mainly because all what I need for experiments were available in our laboratory. In addition, when I prepared this plan, I know each experiment how long time it takes.
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| Strategy for Future Research Activity |
The next step in improvements of the efficacy of IBM on fatigue and muscles atrophy will be performed. At this step of general plan, I will investigate the effect of IBL on muscle atrophy in C57BL/6J mice. Animals will be exposed to two kind of stress inducing muscle atrophy: hindlimb (inactivation) and fasting (hunger). Hindlimb and fasting induce muscle wasting by promoting protein degradation and impaired synthesis. After each stress, power strength will be measured to demonstrate the capacity of IBL on muscles development in IBL-treated mice. Moreover, at the end of experiments, muscles and blood will take from mice and some parameters related to muscle atrophy will determined (muscle weight, muscle ATP, MPS, MPD …etc.).
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| Causes of Carryover |
As I will perform some experiments for next steps of project, I need this amount of budget to ensure the smooth progress of my experiment plan.
I will use mice for in vivo experiment, as well food and bedding. In addition, some ELISA kit will be necessary for some blood and tissue analysis.
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