Utilizing Nature of Allopolyploid in wheat: Comprehensive Identification for Useful Genes through Efficient Construction of Wheat NAM Population

2022 Fiscal Year Final Research Report

• Project/Area Number: 20K15502
• Research Category: Grant-in-Aid for Early-Career Scientists
• Allocation Type: Multi-year Fund
• Review Section: Basic Section 39010:Science in plant genetics and breeding-related
• Research Institution: Kyoto University
• Principal Investigator: Nishimura Kazusa 京都大学, 農学研究科, 助教 (60835453)
• Project Period (FY): 2020-04-01 – 2023-03-31
• Keywords: コムギ / 合成コムギ / NAM集団 / QTL / NGS / MIG-seq

Outline of Final Research Achievements

In this study, I developed a method to rapidly construct a nested association mapping population by taking advantage of the fact that triploid wheat produced by crossing tetraploid wheat with Aegilops tauschii becomes synthetic hexaploid wheat simply by self-pollination. A total of 20,686 florets were emasculated and pollinated with pollen from Aegilops tauschii. As a result, 3,375 triploid seeds could be obtained. Although some of these triploids showed hybrid incompatibility, it was possible to obtain seed of later hexaploid generations from many of the triploid individuals, suggesting that a NAM population could be established quickly by this method. This study also revealed that MIG-seq, which has had limited use in ecological research, is useful for wheat genotyping.

Free Research Field

植物育種学

Academic Significance and Societal Importance of the Research Achievements

本研究によって作出した合成六倍体コムギNAM集団は四倍体コムギにしか分布しない遺伝子を保有する六倍体コムギの遺伝素材としてパンコムギの育種に直接利用することができる。また、MIG-seqがコムギを含むゲノムサイズの大きな作物種において有効に適用できることを明らかにしたことは、本来は遺伝解析に多大なコストのかかるゲノムサイズが大きい作物種の遺伝解析の効率化に資すため、学術的、社会的意義が大きいものと考えている。