Study of infection mechanisms of phytopathogenic bacteria through comprehensive localization analysis of effectors

2022 Fiscal Year Final Research Report

• Project/Area Number: 20K15527
• Research Category: Grant-in-Aid for Early-Career Scientists
• Allocation Type: Multi-year Fund
• Review Section: Basic Section 39040:Plant protection science-related
• Research Institution: Fukushima University
• Principal Investigator: Okano Yukari 福島大学, 食農学類, 准教授 (90734872)
• Project Period (FY): 2020-04-01 – 2023-03-31
• Keywords: イメージング質量分析

Outline of Final Research Achievements

Plant diseases caused by bacteria are difficult to control. Plants have multiple resistance mechanisms against bacteria, but bacteria secrete small proteins called type Ⅲ　effectors into plant cells to suppress the resistance mechanisms. In this study, we performed imaging mass spectrometry on salicylic acid, a plant hormone involved in signaling in plant resistance, and type Ⅲ effectors. Imaging mass spectrometry elucidated the localization of salicylic acid in leaves during bacterial inoculation. I also found that a response similar to the hypersensitive response, which is a strong resistance response, occurs during inoculation with Xanthomonas sp in tobacco.

Free Research Field

植物病理学

Academic Significance and Societal Importance of the Research Achievements

植物の細菌に対する病害応答の際のサリチル酸の組織的な局在を可視化した報告は無く、本研究が初めての報告である。本研究により得られた知見は、細菌に対する病害抵抗性の誘導メカニズムの解明の一端を担うものであり、将来的には植物の抵抗性反応を利用した新規な細菌病の防除法の確立に繋がるものである。