2021 Fiscal Year Final Research Report
Molecular mechanism of poplar miRNAs involved in ectomycorrhiza formation
| Project/Area Number |
20K15553
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| Research Category |
Grant-in-Aid for Early-Career Scientists
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| Allocation Type | Multi-year Fund |
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| Review Section |
Basic Section 40010:Forest science-related
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| Research Institution | The University of Tokyo |
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Principal Investigator |
LI CHAOFENG 東京大学, 大学院農学生命科学研究科(農学部), 特任研究員 (30866687)
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| Project Period (FY) |
2020-04-01 – 2022-03-31
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| Keywords | MiRNA sequencing / Degradome sequencing / Ectomycorrhiza / Populus tomentosa / RT-qPCR / Phenylpropanoid / Cenococcum geophilum |
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| Outline of Final Research Achievements |
We used the miRNA sequencing to identify 51 differentially expressed miRNAs and corresponding 2043 targets in the roots of Populus tomentosa colonized by Cenococcum geophilum. Then we predicted the target sequences of 51 miRNAs with psRNATarget. The prediction of miRNAs-seq and psRNATarget were further confirmed by degradome sequencing and RT-qPCR analysis. GO and KEGG enrichment analysis indicated that the target genes of some differentially expressed miRNAs regulate the ECM symbiosis through the pathways of phenylpropanoid biosynthesis, isoquinoline alkalold biosynthesis, and apoplast, etc. This work indicates that Populus miRNAs and their target genes are an important part of the regulatory network leading to ECM symbiosis development.
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| Free Research Field |
Forest Science
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| Academic Significance and Societal Importance of the Research Achievements |
The research demonstrates that Populus miRNAs and their target sequences are essential to ectomycorrhizal symbiosis, which plays key roles in forest ecosystems and carbon cycle. The results may supply some new insights about how to manage and protect forests.
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