2023 Fiscal Year Final Research Report
Life cycle stage transition in the tiny phytoplankton
| Project/Area Number |
20K15585
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| Research Category |
Grant-in-Aid for Early-Career Scientists
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| Allocation Type | Multi-year Fund |
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| Review Section |
Basic Section 40030:Aquatic bioproduction science-related
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| Research Institution | Fukui Prefectural University |
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Principal Investigator |
Yamada Kazumasa 福井県立大学, 海洋生物資源学部, 助教 (20778401)
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| Project Period (FY) |
2020-04-01 – 2024-03-31
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| Keywords | 植物プランクトン / 生活史 / 世代交代 / パルマ藻 / ボリド藻 |
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| Outline of Final Research Achievements |
Some bolidophytes have two stages in their life cycle: a non-motile stage with a siliceous cell wall, and a naked motile stage. To reveal the physiological differences between the two stages and the mechanism of stage transition, we analyzed the characteristics of the culture strains. Based on the differences in chloroplast autofluorescence intensity between the two stages, we established a method to distinguish the stages using a cell sorter. The strains were classified into two types: strains without the motile stage and strains with the motile stage, suggesting that stage transitions are not induced, at least under the culture conditions used in this study. In addition, the growth rate analysis revealed differences in the adaptation to light intensity between the two stages.
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| Free Research Field |
藻類生理学、細胞生物学
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| Academic Significance and Societal Importance of the Research Achievements |
植物プランクトンの多くは栄養世代が1つで，世代交代をするグループは例外と考えられてきた．しかし近年，多様な系統において世代交代の現象が見出されており，それらの生物が世代交代をする意義や仕組みの解明が求められている．本研究で対象としたパルマ藻は，寒冷海域の基礎生産者としての重要性が示されつつある生物である．本生物には不動性と遊泳性の2つのステージの存在が知られていたものの，両ステージの培養株中の動態や生理学的な違いは不明であった．本研究では培養株中の両ステージの出現動態を長期モニタリングすることに成功し，ステージ間の光環境への適応に違いを見出した．
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