2022 Fiscal Year Annual Research Report
Molecular mechanism of condensin II in the regulation of mitotic chromosome assembly
| Project/Area Number |
20K15723
|
|---|
| Research Institution | Institute of Physical and Chemical Research |
|---|
Principal Investigator |
香西 誠 (YoshidaMakotoMichael) 国立研究開発法人理化学研究所, 開拓研究本部, 協力研究員 (20791453)
|
|---|
| Project Period (FY) |
2020-04-01 – 2023-03-31
|
| Keywords | chromosome / condensin / mitosis / HEAT / phosphorylation |
|---|
| Outline of Annual Research Achievements |
While studies revealing the molecular mechanisms of action of condensin I subunits have emerged, detailed mechanistic activity of condensin II subunits on the assembly of mitotic chromosomes remains largely unexplored. During the research period, recombinant condensin II wild-type and mutant complexes were successfully purified, and the overall roles of non-SMC subunits CAP-D3, CAP-G2, and CAP-H2 were determined. CAP-D3 and CAP-H2 are essential for both chromosome association and chromosome axis formation by the condensin II complex while CAP-G2 is non-essential. During the final fiscal year, regulatory roles of CAP-D3 C-terminal tail region were investigated to find that it has suppressive effects on condensin II through both G2-dependent and G2-independent mechanisms. These findings were published in a peer-reviewed manuscript in the academic journal eLife. Further analysis of the CAP-D3 C-tail has led to determine that mitotic phosphorylation within the region is important to reduce the suppressive effect by the C-tail on the complex. Overall, this study has revealed the molecular mechanisms of action of condensin II non-SMC subunits that fine-tune the complex in the assembly of mitotic chromosomes.
|
