2022 Fiscal Year Final Research Report
Molecular mechanism of condensin II in the regulation of mitotic chromosome assembly
| Project/Area Number |
20K15723
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| Research Category |
Grant-in-Aid for Early-Career Scientists
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| Allocation Type | Multi-year Fund |
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| Review Section |
Basic Section 43010:Molecular biology-related
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| Research Institution | Institute of Physical and Chemical Research |
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Principal Investigator |
Yoshida Makoto (YoshidaMakotoMichael) 国立研究開発法人理化学研究所, 開拓研究本部, 協力研究員 (20791453)
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| Project Period (FY) |
2020-04-01 – 2023-03-31
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| Keywords | condensin / chromosome / mitosis / HEAT / phosphorylation |
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| Outline of Final Research Achievements |
Changes in chromosomal structure that occur during mitosis are essential for successful cell division. Condensin I and II are protein complexes that play important roles in compacting and maintaining chromosome structures. Although the function of condensin I subunits in the assembly of mitotic chromosomes is becoming clear, condensin II subunits remain poorly understood. To elucidate the function of condensin II and its five subunits, the complex was expressed and successfully purified. By testing various mutant complexes, the study was able to elucidate the functional roles of each subunit as well as the similarities and differences between condensin I and II.
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| Free Research Field |
Molecular biology
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| Academic Significance and Societal Importance of the Research Achievements |
Mis-regulation of chromosome organization, primarily mediated by condensin protein complexes, can lead to the development of diseases and cancer. This study elucidates the mechanism of condensin II protein complex to understand how cell division is achieved without errors in chromosome organization.
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