2022 Fiscal Year Annual Research Report
Competitive linear ubiquitin binding in NFkB-signaling
| Project/Area Number |
20K15756
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| Research Institution | Kyoto University |
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Principal Investigator |
Walinda Erik 京都大学, 医学研究科, 助教 (80782391)
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| Project Period (FY) |
2020-04-01 – 2023-03-31
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| Keywords | ubiquitin / ubiquitin binding / M1-linked chains |
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| Outline of Annual Research Achievements |
The research has progressed without major impediments, and various findings have been disseminated through publication. Notably, the molecular dynamics simulations and NMR experiments conducted on ubiquitin chains have yielded compelling outcomes that concur with observations made by other researchers across diverse fields such as X-ray crystallography. Specifically, researchers utilizing different crystallization conditions have reported distinct conformations of the same ubiquitin chain (e.g., K48-linked Ub2). Nevertheless, all of these conformations lie along the same phase-space trajectory as sampled by molecular dynamics. NMR spectroscopy could experimentally verify many of the predictions made by the MD simulations. The primary outcome of this investigation is encapsulated in an article to be published this year. However, several aspects of the study, particularly those concerning chains other than the HOIL-1L NZF domain (such as A20, NEMO, and others), have yet to be made public.
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