2022 Fiscal Year Final Research Report
Single-molecule imaging of dynamic behavior in chromatin structure
| Project/Area Number |
20K15760
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| Research Category |
Grant-in-Aid for Early-Career Scientists
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| Allocation Type | Multi-year Fund |
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| Review Section |
Basic Section 43040:Biophysics-related
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| Research Institution | Tokyo Denki University |
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Principal Investigator |
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| Project Period (FY) |
2020-04-01 – 2023-03-31
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| Keywords | 1分子蛍光イメージング / DNA / マイクロ流体デバイス / 微細加工 / クロマチン |
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| Outline of Final Research Achievements |
This study was undertaken with the aim of developing a system to reconstruct chromatin structure at the single-molecule level and to analyze its dynamic changes. The chromatin structure was shown to be reconstituted by the micrococcal nuclease assay. Next, chromatin-forming DNA was directly observed at the single-molecule level based on the molecular combing method using the developed microscope core unit.
In the future, the dynamic structural variation of chromatin-forming DNA will be analyzed by optimizing the formation conditions of chromatin-forming DNA.
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| Free Research Field |
合成生物学
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| Academic Significance and Societal Importance of the Research Achievements |
本研究では、（１）試験管内でのクロマチン構造の再構成、（２）開発した顕微鏡コアユニットによるDNA１分子の蛍光イメージング、（３）クロマチン形成DNAの１分子蛍光イメージングについて成果を得られた考えている。これらの成果は、クロマチン構造の再構築やその動的変化がゲノム安定化維持に果たす役割を明らかにするための技術が開発される要素技術となったと考えている。
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