Development of a Imaging Method for Chiral Biomarkers Towards Elucidation of Early Stages of Chronic Diseases

2021 Fiscal Year Final Research Report

• Project/Area Number: 20K15972
• Research Category: Grant-in-Aid for Early-Career Scientists
• Allocation Type: Multi-year Fund
• Review Section: Basic Section 47020:Pharmaceutical analytical chemistry and physicochemistry-related
• Research Institution: University of Shizuoka
• Principal Investigator: SUGIYAMA Eiji 静岡県立大学, 薬学部, 助教 (90806332)
• Project Period (FY): 2020-04-01 – 2022-03-31
• Keywords: 質量分析 / キラル / イメージング / イオンモビリティースペクトロメトリー / 誘導体化 / ジアステレオマー

Outline of Final Research Achievements

Recent evidences gain the importance of histopathological analysis of the chiral molecules associated with various diseases. However, selective imaging of an enantiomer in biological tissues is generally challenging. In this study, we developed a new imaging method that combines diastereomer derivatization and ion mobility spectrometry with mass spectrometry imaging. We designed a new derivatization reagent based on chemical structures suitable for the separation. By using the reagent for on-tissue diastereomeric derivatization, we succeeded in visualizing the different distribution of each enantiomer of chiral organic acids (D-, L-2-hydroxyglutaric acid) in mouse tissues.

Free Research Field

質量分析

Academic Significance and Societal Importance of the Research Achievements

本報告時点において質量分析イメージングによるエナンチオ選択的イメージングの実現を報告した例は他に見当たらない。本研究の成果は、種々の疾患に関連する光学異性体を対象とした、新たな病理組織学的解析法の開発に資すると考えられる。