2022 Fiscal Year Final Research Report
Analysis of mitochondrial sheath formation mechanism using KO mice
| Project/Area Number |
20K16107
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| Research Category |
Grant-in-Aid for Early-Career Scientists
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| Allocation Type | Multi-year Fund |
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| Review Section |
Basic Section 48010:Anatomy-related
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| Research Institution | Osaka University |
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Principal Investigator |
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| Project Period (FY) |
2020-04-01 – 2023-03-31
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| Keywords | 精子形成 / 精子ミトコンドリア / nuage |
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| Outline of Final Research Achievements |
Our study used Tsks and Smdr5 KO mice, with a mitochondrial sheath defect. TSKS is localized in the nuage independent of mitochondria. When this nuage disappears due to TSKS deficiency, cytoplasm cannot be removed from spermatozoa at the spermiation step. In other words, TSKS localizes to the testis-specific nuage and functions to remove cytoplasm from sperm during spermiation, when spermatozoa are released from the testis. In addition, we found that Smdr5-deficient mice have an abnormality in sperm mitochondria inner membrane structure.
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| Free Research Field |
生殖生物学
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| Academic Significance and Societal Importance of the Research Achievements |
ヒトでは精子に過剰な細胞質が付着したまま放出されるExcess residual cytoplasmと呼ばれる疾患が存在し，そのため不妊症となる患者が存在する。本研究ではTSKSの機能を明らかにし，TSKSおよびTSKSによって誘導されるTSKS由来nuageが精子から細胞質を除去する上で必須であり，これらのおかげで精子がスリムな流線型を示し，適切な運動性を得られることを明らかにした。本研究を足がかりに不妊症に関する新たな知見が蓄積し，ひいては不妊症治療や男性避妊薬の開発につながることが期待される。
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