Elucidation of 3D dynamics of mitotic organelles in vivo

2021 Fiscal Year Final Research Report

• Project/Area Number: 20K16109
• Research Category: Grant-in-Aid for Early-Career Scientists
• Allocation Type: Multi-year Fund
• Review Section: Basic Section 48010:Anatomy-related
• Research Institution: Kagoshima University
• Principal Investigator: Kusumi Satoshi 鹿児島大学, 医歯学域医学系, 助教 (00758039)
• Project Period (FY): 2020-04-01 – 2022-03-31
• Keywords: 分裂期オルガネラ / 走査電子顕微鏡 / 連続切片SEM法 / 光電子相関顕微鏡法 / オスミウム浸軟法

Outline of Final Research Achievements

In this study, we have visualized three-dimensional (3D) organelle dynamics in differentiated cells during mitosis and revealed that the mitotic Golgi apparatus maintains its laminae structure without fragmentation by using an osmium maceration method and a serial section scanning electron microscopy (SEM) method. Since it is difficult to identify the subdivided Golgi apparatus during mitosis by only morphological observation, we developed novel 3D imaging techniques. Using these techniques, we could accurately identify the mitotic Golgi apparatus and analyze the morphology of its dynamics.

Free Research Field

解剖学

Academic Significance and Societal Importance of the Research Achievements

本研究課題において、これまで培養細胞を中心に解析されてきた分裂期のオルガネラ動態に対し、in vivo環境で分化・成熟した細胞では異なる分裂期ダイナミクスが存在することを示すことができた。また、本研究で開発を進めた最新の3D電子顕微鏡技法は、これらのオルガネラ動態の形態解析に不可欠であるとともに、そのメカニズムを解明する一助となることが明らかであり、当該分野の発展へ大きく寄与することが期待できる。