Roles of LSR in cancer metabolic reprogramming and cell adhesion

2022 Fiscal Year Final Research Report

• Project/Area Number: 20K16196
• Research Category: Grant-in-Aid for Early-Career Scientists
• Allocation Type: Multi-year Fund
• Review Section: Basic Section 49020:Human pathology-related
• Research Institution: Sapporo Medical University
• Principal Investigator: Takasawa Kumi 札幌医科大学, 医学部, 助教 (50359709)
• Project Period (FY): 2020-04-01 – 2023-03-31
• Keywords: LSR / タイト結合 / 細胞接着 / がん代謝 / 子宮頸がん

Outline of Final Research Achievements

Immunohistochemistry with anti-LSR antibodies was performed on surgical material of cervical adenocarcinoma and its staining pattern was evaluated. The results showed a significant difference in the expression of LSR in each cancer component compared to non-neoplastic cervical glandular epithelium and breast duct epithelium. LSR expression-deficient lines generated by the CRISPR-Cas9 system had significantly reduced various tumor malignant potentials compared to control cell lines. Comparative proteomic analysis detected several gene ontology (GO) terms related to cell adhesion. In addition, LSR expression analysis of multiple breast cancer cell lines confirmed that LSR expression was increased by treatment with a couple of drugs.

Free Research Field

腫瘍生物学

Academic Significance and Societal Importance of the Research Achievements

これまでに、子宮頸がんにおけるLSRの異所性高発現および子宮頸がん悪性化におけるLSRの役割解明に関する研究は皆無である。細胞膜表面に異所性高発現するLSRは診断マーカーとして有望であると同時に、分子標的治療のターゲットともなり得ることから、本研究で得られる知見は、分子機序の理解に役立つと共に、LSR高発現がんの治療戦略立案にも寄与すると期待される。