2022 Fiscal Year Final Research Report
New mechanism of teratoma formation associated with GANP function.
| Project/Area Number |
20K16228
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| Research Category |
Grant-in-Aid for Early-Career Scientists
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| Allocation Type | Multi-year Fund |
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| Review Section |
Basic Section 49030:Experimental pathology-related
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| Research Institution | Fujita Health University |
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Principal Investigator |
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| Project Period (FY) |
2020-04-01 – 2023-03-31
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| Keywords | 奇形腫マウスモデル / GANP / 始原生殖細胞 / 奇形腫発生 / 精巣奇形腫 |
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| Outline of Final Research Achievements |
We analyzed the expression of GANP in human testicular postpubertal-type teratomas and established a novel mouse model to reveal the association between GANP and teratomagenesis.
We analyzed 31 cases of human testicular postpubertal-type teratomas and, in all cases, GANP was overexpressed. The aberrant expression was also detected in germ cell neoplasia in situ (GCNIS) accompanied by the teratoma. To further clarify the association between GANP and teratomagenesis, we established a novel teratomagenesis mouse model (CAG-ganp Tg mice). In the GANP－teratoma mice, GANP-overexpressing teratomas were more frequent at the testes and the middle portion of the uterus than has been seen in the previously established mouse models.
In conclusion, GANP is overexpressed in testicular postpubertal-type teratomas and is an essential teratomagenic factor.
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| Free Research Field |
転写共役型DNA傷害
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| Academic Significance and Societal Importance of the Research Achievements |
ヒトiPS細胞を用いた再生医療は疾患の治療に飛躍的な進歩をもたらす可能性を秘めているが、現在では移植による奇形腫の発生が大きな問題となっている。このことを克服するために奇形腫発生制御の研究が世界中で進められている。しかし、従前より使用されている奇形腫好発系のマウス129/Sv系統や129+Ter/Sv系統でも奇形腫の発生率は極めて低く。奇形腫の分子生物学的解析は困難を極めていた．私たちは奇形腫発生のメカニズムにGANPが密接に関係していることを明らかにしたと同時に、CAG-ganp Tgマウスは奇形腫発生率が高く、奇形腫発生を制御するための新しい技術開発にも極めて有用であることが分かった。
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