2021 Fiscal Year Final Research Report
Analysis and identification of virulence genes for B. pertusis infection by in vivo imaging system
| Project/Area Number |
20K16243
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| Research Category |
Grant-in-Aid for Early-Career Scientists
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| Allocation Type | Multi-year Fund |
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| Review Section |
Basic Section 49050:Bacteriology-related
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| Research Institution | Osaka University |
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Principal Investigator |
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| Project Period (FY) |
2020-04-01 – 2022-03-31
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| Keywords | 百日咳 / 生体イメージング / 気管支敗血症菌 |
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| Outline of Final Research Achievements |
The infectious mechanism of Bordetella pertussis, which is causative agent of Pertussis (whooping cough), is still unclear even though various virulence factors are identified. In this study, to reveal its infectious mechanism, we analyzed its virulence factors at once. First, Bordetella bronchiseptica and rats were used as an infectious model and the imaging system was constructed to visualize bacteria in vivo. Next, by in vivo imaging system, the infection ability of mutant strains lacking several virulence genes were evaluated. Our results suggested that B. bronciseptica had ability to infect its host temporarily without known virulence factors.
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| Free Research Field |
細菌学
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| Academic Significance and Societal Importance of the Research Achievements |
本研究では菌の生体内動態を継続的に評価することで、感染初期から後期において、気管支敗血症菌の病原因子が異なるタイミングで機能していることを明らかとした。また感染初期においては未知の因子が感染に関与する可能性を示した。この成果は百日咳菌の感染成立機序を包括的に理解するための端緒となり、将来的には感染成立機序の解明を通して、百日咳を制御する方法の構築につながると考えられる。
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