2021 Fiscal Year Annual Research Report
Development of a human cell-based in-vitro granuloma model to dissect the mechanism of TB reactivation
| Project/Area Number |
20K16258
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| Research Institution | 公益財団法人結核予防会 結核研究所 |
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Principal Investigator |
郭 姿君 (GuoTzーChun) 公益財団法人結核予防会 結核研究所, 生体防御部 病理科, 研究員 (00813065)
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| Project Period (FY) |
2020-04-01 – 2022-03-31
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| Keywords | LTBI / TB reactivation / In-vitro granuloma |
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| Outline of Annual Research Achievements |
To establish the human cell-based in-vitro granuloma model, human donated PBMCs were purchased from the company (Cosmo Bio) and stored in the liquid nitrogen until use. Other necessary reagents for the optimal growth of PBMCs to form granulomas, such as 3D culture gel, human serum for culture medium, and extracellular matrices, enable to mimic in-vivo environments for 3D cell culture applications were all obtained. Additionally, to develop a co-culture system for the dynamic interaction of the formulated granuloma and other immune cells in PBMCs, permeable Transwell Inserts were ordered. Prolonged survivals of granulomas will be used as an indicator for evaluating the feasibility of this co-culture system.
The research was discontinued when the principal investigator was unable to continue the research beyond one year.
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