抗酸菌感染初期におけるCARD9依存的な宿主防御応答の解析

2022 Fiscal Year Annual Research Report

• Project/Area Number: 20K16285
• Research Institution: Fukuoka Dental College
• Principal Investigator: 豊永 憲司 福岡歯科大学, 口腔歯学部, 助教 (90791567)
• Project Period (FY): 2020-04-01 – 2023-03-31
• Keywords: 抗酸菌感染 / 宿主防御 / Th1応答

Outline of Annual Research Achievements

これまでの解析から、Mycobacterium bovis BCGを用いたマウス経気道感染モデルにおいては、CARD9は感染初期の菌排除には積極的に寄与せず、より後期の段階での宿主防御に寄与する可能性を見出している。実際、感染3週間後と9週間後の肺内菌数を検討したところ、感染3週間後では、臓器内菌数に差は認められなかったが、9週間後では、CARD9欠損マウスにおいて臓器内菌数が多い傾向にあった。加えて、感染肺の炎症性サイトカイン量や、縦隔リンパ節細胞を用いた抗原特異的応答の解析からもそのことを支持する結果が得られたため、現在、これらの応答の経時変化を解析するとともに、結核菌感染におけるCARD9の役割についても検討している。

Research Products

• [Journal Article] Pathobiont-responsive Th17 cells in gut-mouth axis provoke inflammatory oral disease and are modulated by intestinal microbiome (2022)
  • Author(s): Nagao Jun-ichi、Kishikawa Sari、Tanaka Honami、Toyonaga Kenji、Narita Yuka、Negoro-Yasumatsu Kanae、Tasaki Sonoko、Arita-Morioka Ken-ichi、Nakayama Jiro、Tanaka Yoshihiko
  • Journal Title: Cell Reports Volume: 40 Pages: 111314～111314
  • DOI: 10.1016/j.celrep.2022.111314
  • Peer Reviewed / Open Access
• [Presentation] Analysis of immune responses in a murine oral candidiasis model (2022)
  • Author(s): Kenji Toyonaga、Emi Kaji、Jun-ichi Nagao、Sonoko Tasaki、Sari Kishikawa、Kanae Negoro-Yasumatsu、Yoshihiko Tanaka
  • Organizer: The 51st Annual Meeting of The Japanese Society for Immunology
• [Presentation] Investigation of Th17-cell mediated immune response against the pathogenic fungus Candida albicans (2022)
  • Author(s): Emi Kaji、Kenji Toyonaga、Sonoko Tasaki、Jun-ichi Nagao、Sari Kishikawa、Mizuko Ikeda、Yosihiko Tanaka
  • Organizer: The 51st Annual Meeting of The Japanese Society for Immunology
• [Presentation] Development of periodontitis mediated by T-cell immune response against periodontal bacteria (2022)
  • Author(s): Jun-ichi Nagao、Sari Kishikawa、Kenji Toyonaga、Emi Kaji、Kanae Negoro-Yasumatsu、Sonoko Tasaki、Yoshihiko Tanaka
  • Organizer: The 51st Annual Meeting of The Japanese Society for Immunology
• [Presentation] Immunological analysis of periodontal disease-induced cognitive disorders in mice (2022)
  • Author(s): Sari Kishikawa、Jun-ichi Nagao、Kenji Toyonaga、Emi Kaji、Kanae Negoro-Yasumatsu、Sonoko Tasaki、Yoshihiko Tanaka
  • Organizer: The 51st Annual Meeting of The Japanese Society for Immunology
• [Presentation] Analysis of immune responses to Candida albicans in a murine oral infection model (2022)
  • Author(s): Toyonaga K、Nagao J、Mizukami K、Tasaki S、Kishikawa S、Kaji E、Negoro K、Tanaka Y
  • Organizer: The 64th Annual Meeting of Japanese Association for Oral Biology
• [Presentation] Investigation of immunological systems regulating the pathogenesis of periodontitis (2022)
  • Author(s): Nagao J、Kishikawa S、Toyonaga K、Kaji E、Negoro-Yasumatsu K、Tasaki S、Tanaka Y
  • Organizer: The 64th Annual Meeting of Japanese Association for Oral Biology
• [Presentation] Neuroimmunological analysis using the mouse model of periodontal disease (2022)
  • Author(s): Kishikawa S、Nagao J、Toyonaga K、Kaji E、Negoro K、Tasaki S、Tanaka Y
  • Organizer: The 64th Annual Meeting of Japanese Association for Oral Biology
• [Presentation] Exploration of antigens from the pathogenic fungus Candida albicans that regulate the pathogenesis of oral candidiasis (2022)
  • Author(s): Kaji E、Toyonaga K、Tasaki S、Nagao J、Kisikawa S、Tanaka Y
  • Organizer: The 64th Annual Meeting of Japanese Association for Oral Biology
• [Presentation] Analysis of host responses against secondary infection of Candida albicans (2022)
  • Author(s): Mizukami K、Toyonaga K、Tanaka Y
  • Organizer: The 64th Annual Meeting of Japanese Association for Oral Biology