2020 Fiscal Year Research-status Report
Studying the dynamics of a novel small RNA (tiRNA) generation in glioma; possible new therapy
| Project/Area Number |
20K16323
|
|---|
| Research Institution | Tohoku University |
|---|
Principal Investigator |
Rashad Sherif 東北大学, 医学系研究科, 助教 (00824088)
|
|---|
| Project Period (FY) |
2020-04-01 – 2023-03-31
|
| Keywords | Glioma / Chemotherapy / tRNA / Cell stress |
|---|
| Outline of Annual Research Achievements |
In order to study the role of tRNA and tiRNA in glioma, I developed different transgenic glioma cell lines with knockdown or overexpression of tRNA modifying enzymes. I discovered that Alkbh1 (m1A demethylase and regulator of tRNA cleavage) influce glioma protein synthesis rate, senescence, proliferation and mitochondrial function. RNA sequencing revealed various gene regulatory networks that were differentially regulated in Alkbh1 overexpressing glioma cells.
Moreover, I continued the focus on tRNA cleavage and oxidative stress and discovered an angiogenin independent non-canonical cleavage pattern of tRNA cleavage in vivo and in various tumor cell lines that possibly regulate cellular oxidative response (Rashad et al, J Cell Phys, 2021)
|
| Current Status of Research Progress |
Current Status of Research Progress
2: Research has progressed on the whole more than it was originally planned.
Reason
Succeded in generating multiple transgenic glioma cell lines for tRNA Modifying enzymes. Transgenic glioma cells show distinct functionality compared to wild-type cells. Interim results reveal an important regulatory role of tRNA modifying enzymes in glioma progression and therapy responses.
|
| Strategy for Future Research Activity |
IN the next phase I will continue the in vitro experimentation to fully analyze the role of tRNA Modifications in glioma cells. Moreover, in vivo experimentation with glioma cell transplantation is planned to evaluate the role of these enzymes on glioma microenvironment
|
