2022 Fiscal Year Research-status Report
Single-cell transcriptomic and epigenomic analysis of young and elder mouse gastric mucosa infected by Helicobacter pylori
| Project/Area Number |
20K16347
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| Research Institution | Hoshi University |
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Principal Investigator |
Liu Yuyu 星薬科大学, 薬学部, 特任助教 (10870462)
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| Project Period (FY) |
2020-04-01 – 2024-03-31
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| Keywords | Gastric mucosa / Gastric stem cells / Aging / Epigenetics / Chromatin accessibility |
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| Outline of Annual Research Achievements |
This year, we conducted and analyzed scATAC-seq data obtained from gastric mucosa of 3-week-old and 20-week-old mice. The results indicate that there are differences in chromatin accessibility in gastric epithelial cells, particularly in stem cells, between the two age groups, while the expression profiles are similar. Additionally, the chromatin accessibility in fibroblasts remained consistent with age, but the expression profiles differed.
Moreover, we established organoids from single pyloric glands and conducted Wnt withdrawal experiment again. Organoids derived from single glands of 3-week-old mice antrum tissue exhibited a more pronunced Wnt-dependency than those derived from bulk glands. In contrast, organoids derived from 20-week-old mice were unaffected by Wnt withdrawal.
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| Current Status of Research Progress |
Current Status of Research Progress
1: Research has progressed more than it was originally planned.
Reason
As per our plan, we have conducted scRNA-seq and scATAC-seq experiments using 3-week-old and 20-week-old mice antrum tissue. Currently, we are in the process of completing the data analysis and starting the preparation for publication.
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| Strategy for Future Research Activity |
We have tested our hypothesis that 1) the elderly gastric mucosa has a decreased number of stem/progenitor cells compared to younger mucosa, and 2) there are differences in the basal DNA methylation of gastric mucosa stem cells between young and old individuals due to aging. Our findings indicate that the cell population remains unchanged, but there are differences in the epigenomic profile between young and old gastric epithelial cells. We will further investigate the potential consequences of these differences and hypothesize that they may be related to epigenetic plasticity.
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