Comprehensive elucidation of a new monocyte subtype with high expression of insulin resistance-inducing factor, resistin.

2023 Fiscal Year Final Research Report

• Project/Area Number: 20K16523
• Research Category: Grant-in-Aid for Early-Career Scientists
• Allocation Type: Multi-year Fund
• Review Section: Basic Section 52010:General internal medicine-related
• Research Institution: Ehime University
• Principal Investigator: Ikeda Yosuke 愛媛大学, 医学部附属病院, 助教 (60866698)
• Project Period (FY): 2020-04-01 – 2024-03-31
• Keywords: レジスチン

Outline of Final Research Achievements

We conducted gene expression and functional analysis of resistin in monocyte-macrophage subtypes. At the current stage, there is acknowledgment of potential technical errors in flow cytometry, thus comprehensive functional integration analysis remains a challenge. However, we have progressed in collecting samples from the general population and have established the infrastructure for sample collection from type 2 diabetes patients and glucose-tolerant volunteers. Utilizing samples from glucose-tolerant volunteers, we have acquired the techniques for classifying monocyte subtypes. Moving forward, we believe this will aid in elucidating the mechanisms of genetic and environmental interactions centered around resistin.

Free Research Field

糖尿病・代謝

Academic Significance and Societal Importance of the Research Achievements

単球・マクロファージのサブタイプ別にレジスチン遺伝子発現・機能解析を行った。現段階ではフローサイトメトリーによる手技の誤差も認められるため、本格的な機能統合解析は課題である。健診を受診した一般住民より検体を採取し、単球細胞を抽出してサブタイプ分類を行った。測定誤差をなくすことで、今後はレジスチンを起点とした遺伝子・環境因子相互作用の機序解明の一助になると考えている。