Comprehensive microbiome analysis of the association between salivary microbiota and metabolic syndrome

2023 Fiscal Year Final Research Report

• Project/Area Number: 20K16559
• Research Category: Grant-in-Aid for Early-Career Scientists
• Allocation Type: Multi-year Fund
• Review Section: Basic Section 52010:General internal medicine-related
• Research Institution: Kyorin University
• Principal Investigator: MIYOSHI Sawako 杏林大学, 医学部, 学内講師 (90846817)
• Project Period (FY): 2020-04-01 – 2024-03-31
• Keywords: 口腔内微生物叢 / Microbiome解析 / 生活習慣病 / 人間ドック / 唾液

Outline of Final Research Achievements

This study aimed to assess the sequential differences in the oral microbiota from healthy populations to metabolic syndrome patients using saliva samples collected at health checkup, which were less influenced by environmental factors such as diet. The subjects were divided into four groups: healthy group, metabolic syndrome group, metabolic-related disease group without obesity, and obesity group without metabolic-related diseases. These groups were evaluated for differences in diversity and composition of saliva microbiota separately for men and women. Comparing the richness and evenness of bacterial species in each group, the male obesity group without metabolic-related disease showed a significant decrease in diversity. Furthermore, significant differences in bacterial composition were found between the male obesity group with diseases and the male metabolic syndrome group with obesity.

Free Research Field

消化器内科

Academic Significance and Societal Importance of the Research Achievements

本研究において、人間ドック受診者という一般人口において、同じ肥満例であっても、糖尿病や高血圧、脂質異常症といった生活習慣病の有無により口腔内細菌叢の変化が生じる可能性が示唆された。この知見は口腔内細菌叢と生活習慣病の病態との関連を示したという学術的意義とともに、採取の容易な唾液検体からの生活習慣病発症リスク評価、モニタリングツール開発という社会的意義につながるものと考えられる。