2022 Fiscal Year Final Research Report
Visualization of breast tumor with its tumor vessel and intratumoral hypoxic region by photoacoustic imaging
| Project/Area Number |
20K16822
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| Research Category |
Grant-in-Aid for Early-Career Scientists
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| Allocation Type | Multi-year Fund |
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| Review Section |
Basic Section 52040:Radiological sciences-related
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| Research Institution | Kyoto University |
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Principal Investigator |
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| Project Period (FY) |
2020-04-01 – 2023-03-31
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| Keywords | 光超音波 / 腫瘍血管 / 腫瘍低酸素領域 |
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| Outline of Final Research Achievements |
The purpose of this study was to visualize mammary tumors, tumor blood vessels, and hypoxic regions within tumors in mice by means of photoacoustic imaging system.
Photoacoustic imaging allows clear delineation of photoacoustic material. Tumor blood vessels are depicted through hemoglobin, one of photoacoustic material. In order to delineate the tumor itself, we tried to prepare an antibody that binds specifically to the tumor with other photoacoustic substances, indocyanine green (ICG) and IRDye, and constructed an experimental system to measure the intratumoral oxygen concentration of cancer-bearing mice using an oxygen concentration measuring device. Since it became difficult to use the photoacoustic imaging system for animals, the acquisition of photoacoustic images and comparison with oxygen concentration data were carried over to the next study.
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| Free Research Field |
乳腺腫瘍学
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| Academic Significance and Societal Importance of the Research Achievements |
動物用光超音波イメージング装置が使用可能となり、本研究で作成した腫瘍特異的な抗体に光音響物質を付加する方法と同様の手法で低酸素マーカーとなる抗体に光音響物質を付加することで、腫瘍と腫瘍血管のみならず、腫瘍内低酸素領域が光超音波イメージング装置で画像化されるようになると考える。その描出結果と腫瘍内酸素濃度の実測結果、腫瘍の免疫染色データ等を３次元的に比較することで、光超音波イメージングにより腫瘍内低酸素領域が適切に画像化されうることが示されれば、腫瘍の悪性化に関与するその他のマーカーにも応用可能となるであろう。これは、さらなる乳癌の個別最適化治療に繋がると思われる。
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