The role of CHOP in intestinal epithelial cells

2022 Fiscal Year Final Research Report

• Project/Area Number: 20K16949
• Research Category: Grant-in-Aid for Early-Career Scientists
• Allocation Type: Multi-year Fund
• Review Section: Basic Section 53010:Gastroenterology-related
• Research Institution: Akita University
• Principal Investigator: Shimodaira Yosuke 秋田大学, 医学系研究科, 助教 (20777982)
• Project Period (FY): 2020-04-01 – 2023-03-31
• Keywords: CHOP / 腸管上皮細胞

Outline of Final Research Achievements

We have generated mice in which CHOP was specifically knocked out in the intestinal epithelium (CHOPΔIEC) and investigated the role of CHOP in the intestinal epithelium in vivo. CHOPΔIEC mice were born normally and showed no significant changes in the intestinal tract growth macroscopically and histologically.
CHOPΔIEC mice reduced DSS-induced colitis compared with that in control mice. Furthermore, in order to elucidate the role of CHOP in the intestinal epithelium, we collected feces, intestinal epithelial cells and extracted DNA, RNA, and protein, and examined the downstream assay of intestinal microbiota, and qPCR, and Western blotting to investigate the mechanism. Results are currently under analysis and will be reported when finished.

Free Research Field

消化器内科学

Academic Significance and Societal Importance of the Research Achievements

小胞体ストレス応答に関わる分子であるCHOPが腸管上皮細胞において腸炎保護的に関わることが明らかとなった。現在解析中であるが、オートファジーやアポトーシス、その他の小胞体ストレス応答関連分子との関わりを明らかにすることでメカニズムが明らかとなる可能性がある。炎症性腸疾患は患者数が増え、難治例も増えている。本研究結果を元に新たな機序の治療法に展開していくことが重要である。また小胞体ストレス応答は細胞維持の基礎となる細胞内機構の一つであり、腸炎だけでなく他疾患との関わりについても発展が期待される。