Functional analysis of SIRT7 in intestinal tissues and exploration of its association with inflammatory bowel disease

2022 Fiscal Year Final Research Report

• Project/Area Number: 20K16961
• Research Category: Grant-in-Aid for Early-Career Scientists
• Allocation Type: Multi-year Fund
• Review Section: Basic Section 53010:Gastroenterology-related
• Research Institution: Kumamoto University
• Principal Investigator: Furuta Yoki 熊本大学, 病院, 特任助教 (00869513)
• Project Period (FY): 2020-04-01 – 2023-03-31
• Keywords: Sirt7 / 腸炎 / 炎症性腸疾患

Outline of Final Research Achievements

To analyze the function of ubiquitously expressed Sir7 in the intestinal tissues, we analyzed the epithelial cells and intestinal myofibroblasts separately, and the results of RNA-seq analysis showed that the gene expression patterns in the epithelium extracted from WT and Sirt7 KO tended to differ. In the analysis of intestinal myofibroblasts, Sirt7 expression was upregulated by TGF-β stimulation. In addition, collagen production upon TGF-β stimulation was found to be different between WT and SIRT7KO cells.
These findings suggest that Sirt7 regulates gene expression in epithelial cells and myofibroblasts and is involved in inflammation and intestinal fibrosis.

Free Research Field

消化器内科学

Academic Significance and Societal Importance of the Research Achievements

本研究はSirt7が腸管炎症や腸管線維化に関与することを示した。Sirt7の機能はNAD依存性であり、NADは加齢によりその活性化が低下する。炎症性腸疾患(IBD)の発症やその活動性が年代によりことなることが報告されており、その原因の一端がSirt7の活性化にある可能性が示唆され、今後のあらたなIBD治療治療戦略をに寄与するものと考えられる。