2022 Fiscal Year Final Research Report
Examining the Influence of Fusobacterium on Colon Carcinogenesis and Progression - Using Organoids from Clinical Specimens.
| Project/Area Number |
20K16963
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| Research Category |
Grant-in-Aid for Early-Career Scientists
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| Allocation Type | Multi-year Fund |
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| Review Section |
Basic Section 53010:Gastroenterology-related
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| Research Institution | Yokohama City University |
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Principal Investigator |
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| Project Period (FY) |
2020-04-01 – 2023-03-31
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| Keywords | 大腸癌 / オルガノイド / フソバクテリウム |
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| Outline of Final Research Achievements |
We have demonstrated that infection of the organoids from human colon cancer with Fusobacterium causes morphological changes and increases the proliferative and migratory capacity of the cells using RT-PCR, fluorescent immunostaining, and Western blotting. The organoids were transplanted into the spleen of mice. Within weeks, liver metastasis appeared. The number and volume of metastases appeared to increase under the influence of Fusobacterium. Further statistical verification is needed by increasing the number of mice. This study suggests that Fusobacterium contributes to colorectal cancer metastasis through promotion of epithelial-mesenchymal transition.
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| Free Research Field |
消化器内科
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| Academic Significance and Societal Importance of the Research Achievements |
国立がん研究センターが公表している2020年のがん統計のまとめによると、大腸がんの死亡数の順位は男性で3位、女性では1位で、今後も死亡数が増えていくことが予想される。大腸癌は他の癌種よりも様々な治療選択枝があるものの十分とは言えず、フソバクテリウムが大腸癌の発癌や進展に与える影響を解明できれば、フソバクテリウムが新しい治療ターゲットになりえる可能性がある。本研究はその一端を示すことができたと考えており、本研究を基礎から臨床に広げていくことが重要であると考えている。
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