Comprehensive gene expression analysis for serrated lesions of the colon to elucidate the risk of malignant transformation.

2022 Fiscal Year Final Research Report

• Project/Area Number: 20K16980
• Research Category: Grant-in-Aid for Early-Career Scientists
• Allocation Type: Multi-year Fund
• Review Section: Basic Section 53010:Gastroenterology-related
• Research Institution: The University of Tokyo
• Principal Investigator: Ohki Daisuke 東京大学, 医学部附属病院, 助教 (40843157)
• Project Period (FY): 2020-04-01 – 2023-03-31
• Keywords: 大腸鋸歯状腺腫 / 網羅的遺伝子発現解析

Outline of Final Research Achievements

Comprehensive expression analysis was performed on endoscopic biopsy specimens of serrated adenoma of the colon (SSA/P). Bioinformatic analysis of the obtained results, including GSEA analysis, showed a strong correlation between SSA/P and MSI-high cancer. These results suggest that SSA/P may provide insight into the mechanism of malignant transformation of SSA/P. Transcriptome analysis showed that SSA/P cells have decreased CDX2 expression from the early stage, and immunostaining revealed that CDX2 expression was clearly decreased from the very early stage of SSA/P. CDX2 is essential for the development, differentiation, and maintenance of intestinal function, and its decreased expression may play an important role in the development of SSA/P. development.

Free Research Field

早期消化管腫瘍に対する内視鏡治療

Academic Significance and Societal Importance of the Research Achievements

大腸鋸歯状腺腫は、近年認識されるようになってきた病変であり、癌化に至る機序や担癌率等、まだ十分に分かり切っていない状況であり、現在様々な研究が行われている。大腸鋸歯状腺腫は発育する過程で、その一部に異形成 (dysplasia)が生じ、さらに発育することで癌に至るとされており、異形成が生じてから癌に至る速度が速い症例も一部にはあると報告されている。今回の結果により、大腸鋸歯状腺腫より異形成が生じて癌化に至る機序の一端が明らかとなった可能性があり、今後の更なる研究が望まれる。